Centrifugation RCF to RPM Converter
Convert centrifugation protocols from RPM to g-force (RCF) or vice versa. Centrifuge results are only reproducible when using RCF due to different rotor radii.
Centrifuge Configuration
Specify rotor radius, input value, and conversion direction.
Relative Centrifugal Force (RCF)
* Based on a rotor radius of 150 mm.
Protocol Best Ptice
Always publish centrifugation protocols in g-force (RCF) rather than RPM to ensure reproducibility across laboratories.
Standard Centrifugation Speed & Force Guidelines Reference
| Application / Target Specimen | Recommended RCF (g-force) | Typical Benchtop RPM (r = 85 mm) | Duration & Temp |
|---|---|---|---|
| Mammalian Cell Pellet (HeLa / Jurkat) | 200 – 300 × g | 1,450 – 1,770 RPM | 5 min @ 4°C or RT |
| Bacterial Cell Harvest (E. coli) | 3,000 – 5,000 × g | 5,600 – 7,250 RPM | 10–15 min @ 4°C |
| Plasmid Mini-prep Column Spin | 11,000 – 16,000 × g | 10,750 – 13,000 RPM | 1 min @ RT |
| Organelle Subftionation (Mitochondria) | 10,000 – 12,000 × g | 10,250 – 11,200 RPM | 15 min @ 4°C |
Calculation Methodology & Equations
RCF Equation
Calculates relative centrifugal force (g-force) using rotor radius in cm and RPM.
RPM Equation
Determines exact tachometer RPM setting needed to achieve target g-force.
Frequently Asked Questions
Why should centrifugation protocols specify RCF (g-force) instead of RPM? ▼
RPM (Revolutions Per Minute) depends entirely on the specific rotor's radius. RCF (Relative Centrifugal Force) measures actual gravitational force ($g$), ensuring reproducibility across different centrifuge models.
How do you calculate RCF from RPM and rotor radius? ▼
Use the formula: $ (g) = 1.118 times 10^{-5} times r_{} times ()^2$, where $r_{}$ is the rotor radius in centimeters.
How do you measure centrifuge rotor radius ($r$)? ▼
Measure the distance from the central drive shaft (spindle) to the maximum radial tip of the centrifuge tube cavity (bottom of bucket or tube) in millimeters or centimeters.
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