Lineweaver-Burk Plot Calculator
Calculate reciprocal values of reaction velocity and substrate concentration to plot the Lineweaver-Burk equation, and determine Vmax, Km, slope, and intercepts.
Calculation Parameters
Specify your stoichiometry inputs.
Calculated Result
Expert Tip
The Lineweaver-Burk plot linearizes the Michaelis-Menten equation, making it easier to determine Km and Vmax from experimental data.
How this Calculator Works
This calculator computes reciprocal kinetics values used in plotting Lineweaver-Burk graphs. Adjust parameters below:
- Maximum Velocity (Vmax): The maximum rate of reaction at saturating substrate levels (Default: 10 μmol/min)
- Michaelis Constant (Km): Substrate concentration at which reaction rate is half Vmax (Default: 5 mM)
- Substrate Concentration ([S]): The amount of substrate present in the mixture (Default: 5 mM)
The results are computed instantly and updated in the results panel on the right.
Formula & Methodology
Where:
- v: Reaction velocity.
- [S]: Substrate concentration.
- Km: Michaelis constant.
- Vmax: Maximum velocity.
Step-by-Step Calculation Example
To compute the Lineweaver-Burk coordinates manually using the default inputs, follow these steps:
- Identify the input parameters. For example:
- Vmax = 10 μmol/min, Km = 5 mM, [S] = 5 mM
- Apply the formula:
1. Calculate v = (10 * 5) / (5 + 5) = 50 / 10 = 5.0 μmol/min 2. Reciprocal velocity 1/v = 1 / 5.0 = 0.20 min/μmol 3. Reciprocal substrate 1/[S] = 1 / 5 = 0.20 mM⁻¹ 4. Y-intercept = 1/Vmax = 1 / 10 = 0.10 5. X-intercept = -1/Km = -1 / 5 = -0.20 6. Slope = Km/Vmax = 5 / 10 = 0.50
- Verify the calculated value which is updated instantly in the results panel on the right.
Frequently Asked Questions
What is a Lineweaver-Burk plot? ▼
A Lineweaver-Burk plot is a double-reciprocal linear graph (1/v vs 1/[S]) derived from the Michaelis-Menten equation.
How do you find Vmax and Km from the plot intercepts? ▼
y-intercept = 1 / Vmax; x-intercept = -1 / Km; slope = Km / Vmax.
How does competitive inhibition alter a Lineweaver-Burk plot? ▼
Competitive inhibitors increase apparent Km (shifting x-intercept right) while leaving Vmax unchanged (y-intercept stays constant).